Figure 1. S6, not ERK, is activated via PI3K pathway
The signaling response of murine bone marrow-derived macrophages to TLR stimulation was investigated under normal signaling conditions and in cells treated with inhibitors of MEK, PI3K, and mTOR. The TLR agonists poly I:C, Pam3, and LPS were used, eliciting signaling responses through the MyD88-independent pathway, the MyD88-dependent pathway, and both pathways (respectively). The signaling response was investigated in macrophages from both C57BL/6 and Myd88-null animals, by staining cells with fluorescently labeled antibodies for IkBa (total), MAPKAPK-2 (phospho-T334), S6 (phospho-S235/236), and ERK (phospho-T202/Y204). The data shown below support a conclusion that S6, not ERK, is activated by the PI3K pathway (Fig. 1). Furthermore, the signaling is seen to be altered in Pam3-stimulated macrophages from MyD88-null animals (Fig. 2).
Figure 1. S6, not ERK, is activated via PI3K pathway
Figure 2. Altered Signaling in MyD88 Knockout
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